Plvx-shrna2
WebSep 4, 2024 · The constructed plasmid was named pLVX-ShRNA2 -Puro-Homo-ATG5-853, and it was packaged and transferred into HEK293 cells. Transfection efficiency was observed at 24 and 72 h after transfection, and the lentivirus pLVX-ATG5 titer was measured as 8 × 10 7 TU/mL. MDA-MB-231 cells were infected, and infection efficiency was … WebJan 30, 2024 · Suppression of IAPP expression by pLVX-ShRNA2-Puro-IAPP exerted opposite effects of increasing pro-apoptotic Bax and caspase-3 gene expression (Figures 5a–c). Upregulation of IAPP also increased ...
Plvx-shrna2
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Web中国微生物资源共享平台,微生物菌种保藏中心,泰斯拓生物提供菌株,atcc菌株,菌株,质粒载体,慢病毒，细菌，真菌,支原体,衣原体,dsmz,ccug,atcc,分子技术试验,相关的技术服务,咨询电 … WebJun 7, 2014 · The TβRII shRNA or negative control sequence was inserted into plvx-shRNA2 vectors (Clontech) using Bam H1 and Eco R1 restriction sites, packed according to the manufacturer’s instructions (Clontech, USA). To make lentiviral particles, ViraPower Lentiviral Expression System (Invitrogen) was used.
WebMar 8, 2024 · The pLVX-shRNA2 lentiviral transfer plasmid was associated with the highest lentiviral titer, while both pLVX-shRNA2 and psi-LVRU6GP plasmids were associated with efficient RNA interference in target cells. WebAfter annealing in vitro, insert pLVX-shRNA-puro construct recombinant plasmid, then enzyme digestion and sequencing analysis. The lentiviral-shRNAs were transient-transfected into K1 cells. TFF3 mRNA and protein levels were test by real-time PCR and western blot respectively in K1 cells at 48h post transient-transfected.
WebpLVX-shRNA2 is an HIV-1-based, lentiviral expression vector designed to express a small hairpin RNA (shRNA) for RNA interference (RNAi) studies. Expression of your shRNA is driven by the RNA Pol III-dependent, human U6 promoter (PU6), located just upstream of the MCS. pLVX-shRNA2 can be used as a plasmid expression vector and transfected into ... WebMar 8, 2024 · The pLVX-shRNA2 lentiviral transfer plasmid was associated with the highest lentiviral titer, while both pLVX-shRNA2 and psi-LVRU6GP plasmids were associated with …
WebSep 22, 2024 · IMPORTANT NOTE: NIH does not independently verify information submitted to the GTR; it relies on submitters to provide information that is accurate and not …
Web与未感染任何病毒的Huh7、Hep3B细胞，感染pLVX-shRNA2空载体的Huh7、Hep38细胞相比，感染携带IGF1R干扰序列慢病毒的Huh7、Hep3B细胞IGF1R mRNA表达水平显著下调，细胞增殖活性明显降低，细胞迁移和侵袭能力明显受到抑制(P均0.01)。结论筛选出1对高效沉默IGF1R基因的siRNA ... hap shaughnessyWebDec 3, 2024 · The pLVX-shRNA2 and pLVX-IRES-ZsGreen1 plasmids (Clontech, USA) were selected as the lentiviral vectors for PC4 knockdown and overexpression, respectively. The target sequences for PC4 knockdown were PC4 sh1 GAAGGAACAGATTTCTGACAT; PC4 sh2 GACAGGTGAGACTTCGAGA; and PC4 sh3 GCAAAGTGCTAATTGATAT. hap shawl historyWebMar 21, 2024 · PLXNA2 (Plexin A2) is a Protein Coding gene. Diseases associated with PLXNA2 include Tetralogy Of Fallot and Schizophrenia . Among its related pathways are … haps helping hands llcWebFeb 1, 2024 · The targets of LACTB shRNA sequences were 5′-CCATGGTTGCTCTTGCCAA-3′ and 5′-GCCATAGTAGAGAGAGCTT-3’. Oligos containing the scrambled or non-targeted shRNAs were cloned into the pLVX-shRNA2-Luc-Puro vector labeled with luciferase. The oligo sequences are displayed in Table S1. Overexpressing or shRNA lentiviruses were … championship transfer news todayWebAug 13, 2024 · Argonaute2 (Ago2) is a key component of the RISC that can directly degrade mRNA by a slicing mechanism 21, 22, we performed RIP assays using the AGO2 antibody to further confirm the interaction... championship transfers 2021/22Web靶向ERRα基因的慢病毒重组载体及其构建方法和应用,深圳市南山区疾病预防控制中心,202410931991.3,发明授权,本发明公开了一种靶向ERRα基因的慢病毒重组载体，包括pLVX‑shRNA1表达载体的基本序列、抗性基因序列、多克隆位点序列和靶向ERRα基因的寡核苷酸链序列；所述寡核苷酸链序列包括正义链序列 ... championship transfersWebAug 9, 2024 · Plasmids pLVX-shRNA2-m and PLVX-mcmv-ZsGreen1 were purchased from Biowit Technologies, Ltd. (China). pLVX-shRNA2-m was first digested with PstI and then filled in with Klenow. The larger fragment was then extracted and cleaved with BamHI. hap shawls free patterns