site stats

P53 activation by knockdown technologies

WebSENP1 interacts with p53 and deSUMOylates p53 in cells and in vitro. Knockdown of SENP1 markedly induced p53 transactivation activity. We further show that SENP1 depletion synergizes with DNA damage-inducing agent etoposide to induce p53 activation and the expression of p21, leading to synergistic growth inhibition of cancer cells. WebBoth commonly used knockdown technologies can thus induce secondary but sequence-specific p53 activation. p53 inhibition could potentially be applicable to other systems to suppress off-target effects caused by other knockdown technologies. ASJC Scopus subject areas Ecology, Evolution, Behavior and Systematics Molecular Biology Genetics

c-Myc inactivation of p53 through the pan-cancer lncRNA MILIP ... - Nature

WebMar 24, 2012 · HIF-2α knockdown, but not HIF-1α knockdown, increases the activation of p53. Finally, inhibition of HIF-2α blocks arsenite-induced proliferation and malignant transformation. WebMay 25, 2007 · Morpholino phosphorodiamidate antisense oligonucleotides (MOs) and short interfering RNAs (siRNAs) are commonly used platforms to study gene function by … gold mining towns https://thequades.com

p53 activation by knockdown technologies. - Abstract - Europe PMC

WebFeb 15, 2024 · LincRNA-p21 knockdown resulted in the reduction between p53 and lincRNA-p21 interaction, facilitated p53 interaction with MDM2, induced NF-κB and STAT3 signaling pathway activation, reversed TAMs phenotype, produced TNF-α to kill tumor cells and thereby exerted anti-tumor function (Fig. 6). Our data indicated that lincRNA-p21 was an … WebFeb 11, 2024 · Aside from p53 activation, MMC causes an extensive DNA damage response and reactive oxygen species activation (21, 22). To activate p53 more specifically and in the absence of DNA damage, we used nutlin-3, an Mdm2 inhibitor that stabilizes p53 ( 23 ), at doses that slow down but do not arrest cell proliferation ( 24 ) (fig. S1, J and K). WebFeb 15, 2011 · Leng has discovered the mechanism by which p53 is inactivated in cancerous cells, allowing tumours to grow. advertisement. "Successful completion of the proposed … headless lady

(PDF) p53 Activation by Knockdown Technologies

Category:p53 Activation by Knockdown Technologies PLOS Genetics

Tags:P53 activation by knockdown technologies

P53 activation by knockdown technologies

Altmetric – p53 Activation by Knockdown Technologies

WebJan 3, 2013 · p53 is activated in response to disruption of the pre-mRNA splicing machinery. In this study, we show that interfering with the splicing machinery results in activation of … WebJan 1, 2016 · Results. Knockdown of the UGT1A proteins by RNAi in human colon cancer cells and knockout of the Ugt1 locus in intestinal crypt stem cells reduces phosphorylated p53 activation and compromises the ability of p53 to control apoptosis. Targeted deletion of intestinal Ugt1 expression in Ugt1 ΔIEC mice represses colon inflammation-induced p53 …

P53 activation by knockdown technologies

Did you know?

WebBoth commonly used knockdown technologies can thus induce secondary but sequence-specific p53 activation. p53 inhibition could potentially be applicable to other systems to … WebMay 25, 2007 · Introduction Morpholino phosphorodiamidate oligonucleotides (MOs) [1] and short inhibitory RNAs (siRNAs) [2] have been instrumental to induce sequence-specific …

WebThe major off-targeting effect is mediated through p53 activation, as detected through the transferase-mediated dUTP nick end labeling assay, acridine orange, and p21 … WebThe major off-targeting effect is mediated through p53 activation, as detected through the transferase-mediated dUTP nick end labeling assay, acridine orange, and p21 …

WebApr 10, 2007 · Both commonly used knockdown technologies can thus induce secondary but sequence-specific p53 activation. p53 inhibition could potentially be applicable to …

WebOct 5, 2024 · Fig. 1: c-Myc activates the pan-cancer lncRNA MILIP to repress p53. a c-Myc bound to MILIP promoter in A549 and MCF-7 cells. An E-box motif, not associated with MYC target genes on Chr22, was...

WebJul 11, 2014 · Here, we found that HRP-3 knockdown regulates the radioresistance of p53-null H1299 cells through a distinctly different molecular mechanism. HRP-3 depletion was sufficient to cause apoptosis of H1299 cells by generating substantial levels of reactive oxygen species (ROS) through inhibition of the Nrf2/HO-1 antioxidant pathway. gold mining towns californiaWebBoth commonly used knockdown technologies can thus induce secondary but sequence-specific p53 activation. p53 inhibition could potentially be applicable to other systems to … headless landshutWebAug 17, 2024 · The tumor suppressor p53 is the most potent cell cycle checkpoint that preserves genome stability by triggering cell cycle arrest, cellular senescence, and/or apoptosis in response to DNA-damaging insults [ 6 ]. headless laptopWebMay 30, 2013 · TRIAP1 knockdown leads to augmented p21 expression before and during p53 activation and slows down cell proliferation. Overall, our research identified multiple factors that function as gene-specific coregulators within the p53 network and reveals several strategies to manipulate the cellular response upon p53 activation. Results headless learning management systemWebApr 10, 2007 · Search worldwide, life-sciences literature Search. Advanced Search headless lag screwsWebDec 3, 2007 · The tumor suppressor p53 is a downstream target of ATM and is required for the induction of apoptosis and senescence upon TRF2 down-regulation. It has been proposed that p53 specifically inhibits DNA synthesis and delays exit from S phase in ALT cells by interfering with telomeric recombination ( Razak et al., 2004 ). headless lady illusionWebThe major off-targeting effect is mediated through p53 activation, as detected through the transferase-mediated dUTP nick end labeling assay, acridine orange, and p21 … gold mining towns in montana