WebSENP1 interacts with p53 and deSUMOylates p53 in cells and in vitro. Knockdown of SENP1 markedly induced p53 transactivation activity. We further show that SENP1 depletion synergizes with DNA damage-inducing agent etoposide to induce p53 activation and the expression of p21, leading to synergistic growth inhibition of cancer cells. WebBoth commonly used knockdown technologies can thus induce secondary but sequence-specific p53 activation. p53 inhibition could potentially be applicable to other systems to suppress off-target effects caused by other knockdown technologies. ASJC Scopus subject areas Ecology, Evolution, Behavior and Systematics Molecular Biology Genetics
c-Myc inactivation of p53 through the pan-cancer lncRNA MILIP ... - Nature
WebMar 24, 2012 · HIF-2α knockdown, but not HIF-1α knockdown, increases the activation of p53. Finally, inhibition of HIF-2α blocks arsenite-induced proliferation and malignant transformation. WebMay 25, 2007 · Morpholino phosphorodiamidate antisense oligonucleotides (MOs) and short interfering RNAs (siRNAs) are commonly used platforms to study gene function by … gold mining towns
p53 activation by knockdown technologies. - Abstract - Europe PMC
WebFeb 15, 2024 · LincRNA-p21 knockdown resulted in the reduction between p53 and lincRNA-p21 interaction, facilitated p53 interaction with MDM2, induced NF-κB and STAT3 signaling pathway activation, reversed TAMs phenotype, produced TNF-α to kill tumor cells and thereby exerted anti-tumor function (Fig. 6). Our data indicated that lincRNA-p21 was an … WebFeb 11, 2024 · Aside from p53 activation, MMC causes an extensive DNA damage response and reactive oxygen species activation (21, 22). To activate p53 more specifically and in the absence of DNA damage, we used nutlin-3, an Mdm2 inhibitor that stabilizes p53 ( 23 ), at doses that slow down but do not arrest cell proliferation ( 24 ) (fig. S1, J and K). WebFeb 15, 2011 · Leng has discovered the mechanism by which p53 is inactivated in cancerous cells, allowing tumours to grow. advertisement. "Successful completion of the proposed … headless lady